Unsupervised learning as a complement to convolutional neural network classification in the analysis of saccadic eye movement in spino-cerebellar ataxia type 2

IWANN es un congreso internacional que se celebra bienalmente desde 1991. Su campo de estudio se centra en la fundamentación y aplicación de las distintas técnicas de Inteligencia Computacional : Redes Neuronales Artificiales, Algoritmos Genéticos, Lógica Borrosa, Aprendizaje Automático. En esta edición han participado 150 investigadores.This paper aims at assessing spino-cerebellar type 2 ataxiaby classifying electrooculography records into registers corresponding to healthy, presymptomatic and ill individuals. The primary used technique is the convolutional neural network applied to the time series of eye movements, called saccades. The problem is exceptionally hard, though, because the recorded saccadic movements for presymptomatic cases often do not substantially di er from those of healthy individuals. Precisely this distinction is of the utmost clinical importance, since early intervention on presymptomatic patients can ameliorate symptoms or at least slow their progression. Yet, each register contains a number of saccades that, although not consistent with the current label, have not been considered indicative of another class by the examining physicians. As a consequence, an unsupervised learning mechanism may be more suitable to handle this form of misclassi cation. Thus, our proposal introduces the k-means approach and the SOM method, as complementary techniques to analyse the time series. The three techniques operating in tandem lead to a well performing solution to this diagnosis problem.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech. Universidad de Granada, Universitat Politècnica de Catalunya, Universidad de Las Palmas de Gran Canaria, Springe

An oleuropein β-glucosidase from olive fruit is involved in determining the phenolic composition of virgin olive oil

Phenolic composition of virgin olive oil is determined by the enzymatic and/or chemical reactions that take place during olive fruit processing. Of these enzymes, β-glucosidase activity plays a relevant role in the transformation of the phenolic glycosides present in the olive fruit, generating different secoiridoid derivatives. The main goal of the present study was to characterize olive fruit β-glucosidase genes and enzymes responsible for the phenolic composition of virgin olive oil. To achieve that, we have isolated an olive β-glucosidase gene from cultivar Picual (OepGLU), expressed in Nicotiana benthamiana leaves and purified its corresponding recombinant enzyme. Western blot analysis showed that recombinant OepGLU protein is detected by an antibody raised against the purified native olive mesocarp β-glucosidase enzyme, and exhibits a deduced molecular mass of 65.0 kDa. The recombinant OepGLU enzyme showed activity on the major olive phenolic glycosides, with the highest levels with respect to oleuropein, followed by ligstroside and demethyloleuropein. In addition, expression analysis showed that olive GLU transcript level in olive fruit is spatially and temporally regulated in a cultivar-dependent manner. Furthermore, temperature, light and water regime regulate olive GLU gene expression in olive fruit mesocarp. All these data are consistent with the involvement of OepGLU enzyme in the formation of the major phenolic compounds present in virgin olive oil

Selection of Tumor-Specific Cytotoxic T Lymphocytes in Acute Myeloid Leukemia Patients Through the Identification of T-Cells Capable to Establish Stable Interactions With the Leukemic Cells: “Doublet Technology”

The relevance of the immune system in cancer has long been studied. Autologous adoptive T cell therapies, based on the use of tumor infiltrating lymphocytes (TILs), have made great progress in recent years for the treatment of solid tumors, especially melanoma. However, further work is needed to isolate tumor-reactive T cells among patients diagnosed with hematologic malignancies. The dynamics of the interaction between T cells and antigen presenting cells (APC) dictate the quality of the immune responses. While stable joints between target cells and T lymphocytes lead to the induction of T cell activation and immune response, brief contacts contribute to the induction of immune-tolerance. Taking advantage of the strong interaction between target cell and activated T-cells, we show the feasibility to identify and isolate tumor-specific cytotoxic T lymphocytes (CTLs) from acute myeloid leukemia (AML) patients by flow cytometry. Using this technology, CTLs bound through T cell receptor (TCR) to tumor cells can be identified in peripheral blood and bone marrow and subsequently selected and isolated by FACS-based cell sorting. These CTLs display higher percentage of effector cells and marked cytotoxic activity against AML blasts. In conclusion, we have developed a new procedure to identify and select specific cytotoxic T cells in patients diagnosed with acute myeloid leukemia.Instituto de Salud Carlos III PFIS-FI12/00189Instituto de Salud Carlos III ISCIII PI14/02074Instituto de Salud Carlos III PI11/02366Instituto de Salud Carlos III PI17/02177European Union (ERDF/ESF, Investing in your future)CIBER CB16/12/0048

Effect of temperature on RNA silencing of a negative-stranded RNA plant virus: Citrus psorosis virus

Citrus psorosis virus (CPsV), genus Ophiovirus, causes a bark scaling disease of citrus. CPsV virions are kinked filaments with three negative-stranded RNA molecules (vRNA) and a 48 kDa coat protein. The effect of temperature on symptom expression, virus accumulation and RNA silencing was examined in sweet orange seedlings (Citrus sinensis) graft-inoculated with three different CPsV isolates and grown in a glasshouse at 26/18°C or 32/26°C (day/night). Most plants kept in the cooler glasshouse showed a shock reaction in the first flush with shoot necrosis, and then moderate to intense chlorotic flecking and spotting in young leaves, whereas plants incubated at 32/26°C did not exhibit shoot necrosis, and young leaf symptoms were milder. Virus titre estimated by ELISA and by northern and dot blot hybridization paralleled symptom intensity, with significantly higher virus accumulation in plants incubated at 26/18°C. The amount of CPsV-derived small RNAs (CPsV-sRNAs) slightly increased at 32/26°C, with the ratio of CPsV-sRNA/vRNA being higher at 32/26°C than at 26/18°C. These results suggest that (i) CPsV infection induces RNA silencing in citrus plants, (ii) symptom intensity is associated with virus accumulation, and (iii) temperature increase enhances the RNA silencing response of citrus plants and decreases virus accumulation.Facultad de Ciencias Exacta

Effect of temperature on RNA silencing of a negative-stranded RNA plant virus: Citrus psorosis virus

Citrus psorosis virus (CPsV), genus Ophiovirus, causes a bark scaling disease of citrus. CPsV virions are kinked filaments with three negative-stranded RNA molecules (vRNA) and a 48 kDa coat protein. The effect of temperature on symptom expression, virus accumulation and RNA silencing was examined in sweet orange seedlings (Citrus sinensis) graft-inoculated with three different CPsV isolates and grown in a glasshouse at 26/18°C or 32/26°C (day/night). Most plants kept in the cooler glasshouse showed a shock reaction in the first flush with shoot necrosis, and then moderate to intense chlorotic flecking and spotting in young leaves, whereas plants incubated at 32/26°C did not exhibit shoot necrosis, and young leaf symptoms were milder. Virus titre estimated by ELISA and by northern and dot blot hybridization paralleled symptom intensity, with significantly higher virus accumulation in plants incubated at 26/18°C. The amount of CPsV-derived small RNAs (CPsV-sRNAs) slightly increased at 32/26°C, with the ratio of CPsV-sRNA/vRNA being higher at 32/26°C than at 26/18°C. These results suggest that (i) CPsV infection induces RNA silencing in citrus plants, (ii) symptom intensity is associated with virus accumulation, and (iii) temperature increase enhances the RNA silencing response of citrus plants and decreases virus accumulation.Facultad de Ciencias Exacta

From pre- to young Planetary Nebulae: radio continuum variability

Searching for variability, we have observed a sample of hot post-AGB stars and young Planetary Nebulae candidates with the Very Large Array at 4.8, 8.4, and 22.4 GHz. The sources had been previously detected in the radio continuum, which is a proof that the central stars have started ionising their circumstellar envelopes and an increase in radio flux with time can be expected as a result of the progression of the ionisation front. Such a behaviour has been found in IRAS 18062+2410, whose radio modelling has allowed us to determine that its ionised mass has increased from 10^{-4} to 3.3 10^{-4} M_sun in 8 years and its envelope has become optically thin at lower frequencies. Different temporal behaviours have been found for three other sources. IRAS 17423-1755 has shown a possibly periodic pattern and an inversion of its radio spectral index, as expected from a varying stellar wind. We estimate that the radio flux arises from a very compact region around the central star (10^{15} cm) with an electron density of 2 10^6 cm^{-3}. IRAS 22568+6141 and 17516-2525 have decreased their radio flux densities of about 10% per year over 4 years. While a linear increase of the flux density with time points out to the progression of the ionisation front in the envelope, decreases as well as quasi-periodic patterns may indicate the presence of unstable stellar winds/jets or thick dusty envelopes absorbing ionising photons.Comment: 9 pages, 5 figures, accepted for publication on MNRA

Comparison of Maldi-Tof with gene sequencing in bacteria isolates from plants belonging to family Rhizobiaceae

Comunicaciones a congreso

Detection of metabolites in Flor de Mayo common beans (Phaseolus vulgaris L.) and their response to inoculation with Trichoderma harzianum

Seed germination involves complex processes where plant growth-promoting substances are released into the growth medium, mainly involving pathways that start in tryptophan (TRP) and end in the formation of indole-3-acetic acid (IAA). Among them, four are known as TRP-dependent and a fifth as TRP-independent. Some compounds were observed during germination of bean seeds that involved at least three IAA synthesis pathways and that kynurenine (KYN) is the first detected metabolite which is found in greater concentration. It was followed by tryptamine (TAM), TRP and IAA. The results of Trichoderma harzianum inoculation in greenhouse tests showed variability in Flor de Mayo beans seedlings in response to physiological level and production parameters. The effect of Trichoderma in Flor de Mayo common bean showed that strain 802 had a significant effect on the development of the height of bean seedlings, and that the 812 strain showed significant effect on the development of root bean seedlings. Increased growth of stem and root caused by Trichoderma strains in Flor de Mayo beans involves beneficial effects of inoculation on plant growth and development parameters and can be taken as they were a measure of survival and development of seedlings.Key words: Biofertilizers, indole-3-acetamide, tryptophan,indole-3- acetic acid, kynurenine